Regenerative Competence in root explants of Cattleya hybrid, an endangered genera: A study in vitro

The regenerative potential in the root explants of in vivo grown Cattleya ’Almakee’ plants largely depend on the location of isolated explants from the main root and on the level&type of the growth regulators.Regeneration is affected by polarity all along the root axis of root.The regeneration is of basipetal gradient.The proximal explants responded to the presence of cytokinins(BAP/KN)in Mitra et al.,1976 medium.The effect of cytokinins was accentuated in the additional presence of NAA,whereas the distal explants failed to regenerate.The regenerated plantlets were accilimatized &transfered to pots filled with moss, pine bark, brick &charcoal pieces (2:4:1:1) with 90% survival.